NanoLC-MS/MS 2 | 액체크로마토그래피 고분해능 오비트랩 질량분석기
| Laboratory/Field | |||
|---|---|---|---|
| Model | Q-Exactive plus | ||
| Maker | Thermo Fisher | ||
| Technician | Eun-jung Han | ||
| Contact | 052-217-2640 / ejhan@unist.ac.kr | ||
| Status for Reservation | 가능 | ||
| Reservation Unit | 2hr | Maximum Time (per day) | 24hr |
| Open(~ago) | 5일전 | Cancel (~ago) | 1일전 |
| Equipment location | 108동 지하 103호(Bldg. 108 Room B103) | ||
-
Description
- Increased resolution up to 140,000 FWHM when analyzing samples in complex matrices, eliminating isobaric interference for highly reliable results
- Compounds can be accurately identified with a mass accuracy of better than 1ppm in AIF (All Ion Fragmentation) scanning mode.
- Fast scanning at 12Hz and spectral multi-detection suitable for UHPLC applications
- Maximum information obtainable through rapid polarity switching
- Mass range up to 6,000 m/z improves detection of singly charged small molecules and biomolecules
- The dynamic range between magnetic intensity scans is over 10,000 times, and the sensitivity is high at the femtogram level, allowing detection of trace levels and high abundance compounds within the same scan -
Specifications
유형 - Quadrupole-Orbitrap LC-MS
동적 범위 - >5000:1
질량 범위 - m/z 50 &sim, 6000
질량 정확도 - 내부 보정: <1ppm RMS, 외부 보정: <3ppm RMS
스캔 속도 - Up to 12 Hz
극성 전환 - 분해능 35,000에서 전체 주기 1회 <1초(전체 스캔 양극 모드 1회 + 전체 스캔 음극 모드 1회)
민감도 - 모든 MS: 500fg 부스피론 on-column S/N 100:1 SIM: 30fg 부스피론 on-column S/N 100:1
Max. Resolution - Up to 240,000 at m/z 200
-
Applications
- 저분자~고분자(단백질)분석을 통한 proteomics 연구
- 유/무기물 질량측정
- Isotope pattern 측정