Photoactivated localization microscopy_Super Resolution | 초고해상도 현미경(PALM)
| Laboratory/Field | |||
|---|---|---|---|
| Model | ELYRA P.1 | ||
| Maker | Carl Zeiss | ||
| Technician | Jin-Hoe Hur | ||
| Contact | 052-217-4161 / jhhur@unist.ac.kr | ||
| Status for Reservation | 가능 | ||
| Reservation Unit | 1hr | Maximum Time (per day) | 무제한 |
| Open(~ago) | 15일 | Cancel (~ago) | 3일전 |
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Description
PALM, Detection with an effective resolution down to 20 nm can show substructure and patterns where conventional microscopy will reveal “merely” co-localization. This can achieve effective lateral resolutions down to 20 - 30 nm and 50 - 80 nm axially.
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Specifications
• Body : ZEISS Axio Observer Z.1
• Objectives : 10 x, 20 x, 40 x (oil), 63 x (oil), 100 x (oil)
• Transmitted Light Source : 100 W Halogen
• Fluorescence Light Source : 120 W Metal Halide
• Observation : Bright Field, TIRF, Fluorescence, Superresolution Imainging
• Laser : 405, 488, 561, 640 nm
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Applications
• Two-Color SR Imaging
• PALM use photoswitchable proteins; excellent Z capture range
• Resolution : XY 20 nm, Z 50 nm
• Map protein localization onto a structural context
• Track many molecules and retrieve diffusion behavior