LC/MS/MS 3 | 초고성능액체크로마토그래피 고분해능질량분석기
| 연구실/분야 | |||
|---|---|---|---|
| 모델명 | ACQ. I-Class PLUS System & Xevo G3 QTof | ||
| 제조사 | Waters | ||
| 담당자 | 서현경 | ||
| 연락처 | 052-217-4183 / seohk26@unist.ac.kr | ||
| 예약 가능여부 | 가능 | ||
| 예약단위 | 1hr | 1일최대예약시간 | 12hr |
| 예약Open(~일 전) | 5일전 | 예약취소불가(~일 전) | 1일전 |
| 장비위치 | 102동 B111호 (Bldg.102, Room.B111) | ||
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Description
The Xevo™ G3 QTof integrates StepWave™ XS ion optics, an XS Collision Cell, and QuanTof™ technology to deliver exceptional robustness, sensitivity, and selectivity. It offers high dynamic range, excellent mass accuracy, and fast analysis speed, ensuring the highest quality in both qualitative and quantitative data. Engineered for molecular characterization, the StepWave™ XS ion optics enable efficient transmission of low m/z and labile species, expanding compound coverage and analytical fidelity, while still maintaining accurate analysis of high-mass biomolecules. As a high-performance benchtop mass spectrometer, the Xevo G3 QTof is designed for detailed characterization and precise quantification across a broad range of molecules. The system supports not only traditional MS and MS/MS acquisition modes, but also data-independent acquisition (DIA) modes such as MSE and SONAR™, delivering comprehensive precursor and fragment ion data in a single run. The instrument also includes FastDDA mode for rapid, automated MS/MS acquisition, and Tof-MRM with adaptive intensity control for enhanced targeted quantification. Furthermore, compatibility with a wide range of ion sources and inlets, under the control of MassLynx™ or waters_connect™ software, provides the flexibility to adapt to evolving analytical requirements.
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Specifications
[Hardware Specifications]
- Ionization Sources: ZSpray™ dual-orthogonal API sources with multiple options (ESI/APCI)
- Ion Optics: StepWave™ XS segmented quadrupole optics for enhanced sensitivity with labile/low-mass compounds
- Mass Analyzers: Quadrupole (MS1) with high resolution and stability oaTof (MS2) with mass range up to m/z 100,000
- Resolving power >40,000 FWHM
- Collision Cell: XS Collision Cell with software-programmable collision energy control
- Detector: Ultra-fast electron multiplier + hybrid analog-to-digital converter for high sensitivity and quantitative performance
- Vacuum System: Differentially pumped, automated with turbomolecular and oil-free backing pumps
[Performance Specifications]
- Acquisition Modes: Full MS, MS/MS, UPLC-MSE, FastDDA, Tof-MRM, SONAR™, ionization switching (ESCi)
- Mass Range
TOF: m/z 20 – 100,000
Quadrupole: m/z 20 – 16,000 (non-resolving), m/z 20 – 4,000 (resolving)
- Mass Accuracy: Better than 1 ppm RMS (with lock mass)
- Dynamic Range: At least 4 orders of magnitude (up to 5 in ToF-MRM mode with adaptive intensity control)
- Resolution
Resolution Mode: >40,000 FWHM
Sensitivity Mode: >30,000 FWHM
- Sensitivity
MS (ESI+): >120,000 cps at m/z 556
MS (ESI-): >165,000 cps at m/z 503
MS/MS: >7,500 cps for [Glu1]-Fibrinopeptide B
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Applications
- Low molecular weight compound analysis (structural elucidation and quantification of drug candidates, impurity profiling)
- High molecular weight and synthetic compounds (polymer composition verification, characterization of composite materials)
- Pharmacokinetics and metabolite analysis
- Drug-drug interaction studies
- Identification and quality control of natural products
- Detection of additives, impurities, and contaminants in food
- Pesticide, endocrine disruptor, and toxic compound analysis
- Precision analysis of metabolites and lipids in cells and body fluids
- Characterization of antibody and protein therapeutics
- Biomarker discovery and validation