Photoactivated localization microscopy_Super Resolution | 초고해상도 현미경(PALM)
| 연구실/분야 | |||
|---|---|---|---|
| 모델명 | ELYRA P.1 | ||
| 제조사 | Carl Zeiss | ||
| 담당자 | 정홍찬 | ||
| 연락처 | 052-217-4181 / jsindom@unist.ac.kr | ||
| 예약 가능여부 | 가능 | ||
| 예약단위 | 1hr | 1일최대예약시간 | 무제한 |
| 예약Open(~일 전) | 15일 | 예약취소불가(~일 전) | 3일전 |
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Description
PALM, Detection with an effective resolution down to 20 nm can show substructure and patterns where conventional microscopy will reveal “merely” co-localization. This can achieve effective lateral resolutions down to 20 - 30 nm and 50 - 80 nm axially.
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Specifications
• Body : ZEISS Axio Observer Z.1
• Objectives : 10 x, 20 x, 40 x (oil), 63 x (oil), 100 x (oil)
• Transmitted Light Source : 100 W Halogen
• Fluorescence Light Source : 120 W Metal Halide
• Observation : Bright Field, TIRF, Fluorescence, Superresolution Imainging
• Laser : 405, 488, 561, 640 nm
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Applications
• Two-Color SR Imaging
• PALM use photoswitchable proteins; excellent Z capture range
• Resolution : XY 20 nm, Z 50 nm
• Map protein localization onto a structural context
• Track many molecules and retrieve diffusion behavior